ABSTRACT
Objective To investigate the effects of Akkermansia muciniphila (AKK) on azomethane-oxide (AOM)/glucan sodium sulfate (DSS)-induced inflammatory colorectal cancer mouse model and intestinal stem cells. Methods AOM/DSS-induced mouse models of inflammatory-associated colorectal cancer were randomly divided into three groups, namely, model, AKK and aspirin groups, based on different administration of drugs by gavage. The tumor number, size, distribution, and burden were observed 10 weeks after intervention. Immunohistochemical method was used to analyze the expressions of Ki67 and Lgr5 proteins, which are utilized to characterize tumor malignancy and stem cells. The mRNA expressions of Lgr5, CD133, Nanog, and ALDH1 were detected by qRT-PCR. Results Compared with those of the model group, the tumor number, size, and burden of the AKK group were significantly reduced (P < 0.01). The expressions of Ki67 and Lgr5 in the AKK group of tumor tissues were significantly decreased (P < 0.05), and the mRNA expressions of CD133, Nanog and ALDH1 were significantly down-regulated. Conclusion AKK is effective against AOM/DSS-induced colitis-associated colorectal cancer in mice, and its mechanism of action may be closely related to colorectal stem cell activity.
ABSTRACT
Objective To investigate the effect of interventional embolization on the clinical outcome and cognitive function of patients with anterior communicating artery aneurysm rupture .Methods The data of 120 patients with anterior communicating artery aneurysm rup-ture in our hospital from January 2016 to January 2017 were retrospectively analyzed ,in which 71 cases were treated by the spring coil emboli-zation,21 cases received balloon-assisted coiling,23 cases received stent-assisted coil embolization .At the same time,50 healthy people were collected as control group .The preoperative , postoperative cognitive function and the clinical effect of the patients were evaluated .Results Coil embolization group completed embolism in 29 cases,40 cases of most embolism ,2 cases of partial embolization;balloon-assisted coiling group completed embolism in 16 cases,4 cases of most embolism ,1 cases of partial embolization;17 cases of stent assisted coil embolization group completed embolism ,3 cases of most embolism ,3 cases of partial embolism .There was statistically significant difference in embolization rate of coil embolization group(χ2 =6.8862,P=0.0320),balloon-assisted coiling group(χ2 =15.900,P=0.0004) and stent assisted coil embolization group(χ2 =7.280,P=0.0262).After the treatment,the difference in cognitive function of coil embolization group (24.0 ± 0.2) and balloon-assisted coiling group(24.3 ±0.2) was statistically significant (t=86.0386,P=0.0000);the difference between coil embolization group(24.0 ±0.2) and balloon-assisted coiling group(24.3 ±0.2) was statistically significant(t=46.3848,P=0.0000);the difference between balloon-assisted coiling group(24.3 ±0.2) and stent assisted coil embolization group (21.5 ±0.2) points was statisti-cally significant(t=52.1002,P=0.0000).Conclusion Different interventional embolization techniques can improve the cognitive func-tion of patients with ruptured anterior communicating artery aneurysm ,which has more obviously effect on the cognitive function of patients with stent assisted coil embolization .